Hi.

I’m Victoria, and this site mostly has my sewing projects and science writing, with a sprinkling of older poetry & etc.

I’m a prairie kid, parent of two, celiac, and like lane swimming. I love to research. I did my undergrad in physics & English with a math minor. I’m now doing a Master’s in economics.

A note about the blog title: in math and physics, the prefix eigen means one's own. It comes from the german, but mostly I always liked thinking about a particle's eigenvalues, and thought I might apply the same thought to my excursions.

Prion research insight into mad cow, chronic wasting disease

Prion research insight into mad cow, chronic wasting disease

deer_prion_bound_to_pom1-1.png

Putting a stop to deadly brain wasting diseases

Chronic Wasting Disease threatens populations of mule deer, white tail deer and elk throughout the prairies, and scientists are working hard towards finding a solution.

Chronic Wasting Disease (CWD) in deer and elk, like Bovine Spongiform Encephalopathy (BSE) in cattle, is caused by the misfolding of a mysterious class of proteins known as prions. While researchers are not clear on their function in mammals, they do know that misfolded prions can cause devastating transmissible infectious diseases.

healthy male mule deer

“The devastating effects that CWD has on these poor deer is just unbelievable,” says University of Alberta researcher Dr. Michael N.G. James. “Prion diseases cause progressive dementia, debilitating motor dysfunction, and eventually death.”

James and his colleagues are using synchrotron techniques to develop three-dimensional structures of the prion molecules in order to see the differences between how the healthy protein folds and how the unhealthy forms might develop.

“Our ultimate goal is to develop small molecules that would bind to the prion proteins in their normal form and prevent them from being converted into the infected form,” says James.

A treatment for prion disease would be a vital safeguard for Canadian farmers, and prevent an economic crisis like that caused by the first Canadian case of BSE in May 2003, when the discovery of a single infected cow caused the slaughter of hundreds of cattle in Alberta, Saskatchewan and British Columbia to prevent the spread of the infection.

Canadian producers lost between $8 billion and $10 billion, and families with beef cattle operations saw their income decline 33% in 2003.

The first step in developing a molecule to block prion misfolding and disease is to understand the prion’s function; then, a molecular structure can be honed to block misfolding.

Prion protein

To get this 3-D information, the team uses crystallized versions of the proteins bound to the molecules they are interested in. Then they use X-ray diffraction methods to gather complex information about their 3-D structures. Individual and isolated prions have been notoriously difficult to crystallize, but James’ team has overcome the difficulty by pairing prions with cognate antibodies, providing stable crystals and clear structural data.

James’ team has determined the 3-D structures of four prion proteins – one each from cattle, deer, elk, and Syrian hamster – thereby identifying subtle differences in their folding.  These differences could help to explain differences in disease transmissibility between different strains, while their similarities point to potential paths for treatment.

This post first appeared as a science highlight for the Canadian Light Source. To read the relevant research: Baral, Pravas Kumar, Mridula Swayampakula, Adriano Aguzzi, and Michael NG James. "X-ray structural and molecular dynamical studies of the globular domains of cow, deer, elk and Syrian hamster prion proteins." Journal of structural biology 192, no. 1 (2015): 37-47.
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